SPECTROstar Omega Microplate Reader from BMG LABTECH

Product Specs
ItemSPECTROstar Omega Microplate Reader
CompanyBMG LABTECH
Price Get Pricing Get Quote for this Product
Catalog NumberSPECTROstar Omega
RangeUp to 45 °C or 65 °C
Well ShapeAll
WellsUp to 1536
Wavelength(s)220 - 1000 nm
Measurement TimeDependent on Test Definition
Plate Formats6 - 1536 Well Plates
Light SourceHigh Energy Xenon Flash Lamp
DetectorCCD spectrometer detector
Resolution1 , 2 , 5 and 10 nm
Accuracy< 1% at 2 OD
Precision±0.005 OD for 0 - 2 OD Range
DetectionSpectrometer with CCD
Dimensions(WxDxH) 44 x 48 x 30 cm (17 x 19 x 11 inches)
ApplicationsAbsorbance (UV-Vis)
Sample Type6- to 1536-well plates

Specifications/Features

Temperature incubation, Shaking, Injectors, Well scan

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BMG LABTECH

BMG LABTECH Allmendgruen 8

Ortenberg 77799

Germany

Phone: +49 781 96968-0

Email: sales@bmglabtech.com

Company Profile

Website: http://www.bmglabtech.com

(1) Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell LineInt J Mol SciDecember 5, 2023Seyed Hesamoddin Bidooki, Javier Sánchez-Marco, Roberto Martínez-Beamonte, Tania Herrero-Continente, María A. Navarro, María J. Rodríguez-Yoldi, Jesús Osadamanufacturer’s instructions using a Quick-RNATM MiniPrep kit (Zymo Research, CA, USA). A spectrophotometer (SPECTROstar®, Omega, BMG Labtech, Ortenberg, Germany) was used to measure the amount of RNA based on the absorbance ratio

(2) Damaging effects of UVA, blue light, and infrared radiation: in vitro assessment on a reconstructed full-thickness human skinFront Med (Lausanne)December 1, 2023Paula Montero, Inés Roger, Javier Milara, Julio Cortijothe NHEK cells or 2 h in the 3D skin models. Absorbance was measured at 572 nm using the plate reader Spectrostar Omega (BMG LabTech, Biogen, Madrid, Spain). Data were normalized to control values. The cytotoxicity assay

(3) SMYD3 Modulates AMPK-mTOR Signaling Balance in Cancer Cell Response to DNA DamageCellsNovember 17, 2023Martina Lepore Signorile, Paola Sanese, Elisabetta Di Nicola, Candida Fasano, Giovanna Forte, Katia De Marco, Vittoria Disciglio, Marialaura Latrofa, Antonino Pantaleo, Greta Varchi, et al.determined by a luciferase/luciferin reaction. The generated luminescence was measured using a luminometer (SPECTROstar Omega, BMG LABTECH, Ortenberg, Germany). Each data point was collected in triplicate. 2.7. In Silico Methylation

(4) Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidensInternational Journal of Molecular SciencesNovember 10, 2023Sofia A. Shilova, Ilya O. Matyuta, Elizaveta S. Petrova, Alena Y. Nikolaeva, Tatiana V. Rakitina, Mikhail E. Minyaev, Konstantin M. Boyko, Vladimir O. Popov, Ekaterina Yu. Bezsudnova9.0, at 30 °C in microtiter plates (UV-Star, Greiner Bio-One GmbH, Frickenhausen, Germany) using a SPECTROstar Omega plate reader (BMG Labtech, GmbH, Ortenberg, Germany). The rate constants of the half-reactions were

(5) Ploidy evolution in a wild yeast is linked to an interaction between cell type and metabolismPLOS BiologyNovember 9, 2023Johnathan G. Crandall, Kaitlin J. Fisher, Trey K. Sato, Chris Todd HittingerMedium containing maltose or glucose at a final dilution factor of 10−4. Plates were incubated on a SPECTROstar Omega plate reader (BMG Labtech) equipped with a microplate stacker, and OD600 was measured every hour. Raw

(6) SMYD3 Modulates the HGF/MET Signaling Pathway in Gastric CancerCellsOctober 18, 2023Katia De Marco, Martina Lepore Signorile, Elisabetta Di Nicola, Paola Sanese, Candida Fasano, Giovanna Forte, Vittoria Disciglio, Antonino Pantaleo, Greta Varchi, Alberto Del Rio, et al.incubated at 37 °C in a humidified incubator for up to 1 h. The luminescent signal was read using a SPECTROstar Omega microplate reader (BMG Labtech, Ortenberg, Germany). Cell death was assessed by counting. Briefly, AGS and NCI-N87 GC

(7) An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemicsNew BiotechnologySeptember 25, 2023Shayan G. Borhani, Max Z. Levine, Lauren H. Krumpe, Jennifer Wilson, Curtis J. Henrich, Barry R. O’Keefe, Donald C. Lo, G. Sitta Sittampalam, Alexander G. Godfrey, R. Dwayne Lunsford, et al.After 3 d incubation, luciferase reagent was added (PerkinElmer, ) and expression was quantitated (Spectrostar Omega plate reader – BMG Labtech, Ortenberg, Germany). After normalization (absence of GRFT = 100%), dose-response

(8) miR-369-3p Modulates Intestinal Inflammatory Response via BRCC3/NLRP3 Inflammasome AxisCellsAugust 31, 2023Viviana Scalavino, Emanuele Piccinno, Anna Maria Valentini, Nicolò Schena, Raffaele Armentano, Gianluigi Giannelli, Grazia SerinoThe color reaction was stopped with 2N H2SO4, and the optical density was detected at 450 nm using SPECTROstar Omega Spectrometer (BMG Labtech, Ortenberg, Germany). 2.7. Immunofluorescence Following transfection and

(9) Metabolic Alterations Caused by Simultaneous Loss of HK2 and PKM2 Leads to Photoreceptor Dysfunction and DegenerationCellsAugust 10, 2023Eric Weh, Moloy Goswami, Sraboni Chaudhury, Roshini Fernando, Nick Miller, Heather Hager, Sarah Sheskey, Vikram Sharma, Thomas J. Wubben, Cagri G. Besirliconsisting of 50 mM Tris-HCl (pH 7.4), 100 mM KCl, and 5 mM MgCl2 as previously described [4,22,31]. A SPECTROstar Omega plate reader (BMG LABTECH Inc., Cary, NC, USA) was used to measure absorbance at 340 nm. The included

(10) The Redox-Active Manganese(III) Porphyrin, MnTnBuOE-2-PyP5+, Impairs the Migration and Invasion of Non-Small Cell Lung Cancer Cells, Either Alone or Combined with CisplatinCancers (Basel)July 27, 2023Rita B. Soares, Rita Manguinhas, João G. Costa, Nuno Saraiva, Nuno Gil, Rafael Rosell, Sérgio P. Camões, Ines Batinic-Haberle, Ivan Spasojevic, Matilde Castro, et al.were resuspended with a solution of 96% EtOH/1% acetic acid. Absorbance was measured at 595 nm with a SPECTROstar OMEGA microplate reader (BMG Labtech, Offenburg, Germany). Absorbance values of the control cells were defined as 100% cell

(11) Engineered reporter phages for detection of Escherichia coli, Enterococcus, and Klebsiella in urineNature CommunicationsJuly 20, 2023Susanne Meile, Jiemin Du, Samuel Staubli, Sebastian Grossmann, Hendrik Koliwer-Brandl, Pietro Piffaretti, Lorenz Leitner, Cassandra I. Matter, Jasmin Baggenstos, Laura Hunold, et al.microplate sealing film (AxygenTM) and OD600 quantified every five minutes at 30 °C for 8 h using a Spectrostar Omega or Spectrostar Nano plate reader (BMG Labtech). Uninfected bacterial cultures and medium were used as

(12) Enhancing bacteriophage therapeutics through in situ production and release of heterologous antimicrobial effectorsNature CommunicationsJuly 20, 2023Jiemin Du, Susanne Meile, Jasmin Baggenstos, Tobias Jäggi, Pietro Piffaretti, Laura Hunold, Cassandra I. Matter, Lorenz Leitner, Thomas M. Kessler, Martin J. Loessner, et al.sealing film (AxygenTM) and OD600nm was quantified every 5 min at 30 °C using a spectrophotometer (SPECTROstar Omega or SPECTROstar Nano, BMG Labtech). Uninfected bacterial dilutions were used as growth controls, and

(13) Understanding the role of GRE3 in the erythritol biosynthesis pathway in Saccharomyces uvarum and its implication in osmoregulation and redox homeostasisMicrobial BiotechnologyJuly 14, 2023Sonia Albillos‐Arenal, Romain Minebois, Amparo Querol, Eladio Barriowas followed by measuring the OD600 in Stacker Microplate Handling System attached to plate readers SPECTROstar Omega (BMG LABTECH). The OD600 results were analysed by the Growth Curve Analysis Tool (GCAT) (Bukhman et

(14) Comparative genomics of infective Saccharomyces cerevisiae strains reveals their food originScientific ReportsJune 27, 2023Miguel Morard, Laura Pérez-Través, Carla Perpiñá, María Lairón-Peris, María Carmen Collado, Roberto Pérez-Torrado, Amparo Queroldistilled water, and adjusted to an OD600 of 4. The experiment was performed in 48 microwell plates and a SPECTROstar Omega 470 (BMG Labtech, Offenburg, Germany). In each microwell, 20 µL of the cell culture and 380 µL of the

(15) Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root TubersPharmaceuticals (Basel)June 1, 2023Maryam Malmir, Katelene Lima, Sérgio Póvoas Camões, Vera Manageiro, Maria Paula Duarte, Joana Paiva Miranda, Rita Serrano, Isabel Moreira da Silva, Beatriz Silva Lima, Manuela Caniça, et al.purple crystals formed prior to measuring absorbance at 570 nm using a microplate spectrophotometer (SPECTROstar Omega; BMG LabTech, Ortengerg, Germany). The results are expressed as a percentage relative to the solvent

(16) Cold Atmospheric Plasma Exerts Antimicrobial Effects in a 3D Skin Model of Cutaneous CandidiasisAntibiotics (Basel)May 19, 2023Sarah Fink, Michael Fischer, Sebastian Spange, Oliver Beier, Kerstin Horn, Jörg Tittelbach, Cornelia Wiegandmanufacturer’s recommendations. The optical density at 490 nm was measured using a plate photometer (SPECTROstar Omega, BMG Labtech GmbH, Ortenberg, Germany). The fold LDH release was calculated relative to the control. 4.5.

(17) Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor ApoptosisPharmaceuticals (Basel, Switzerland)May 6, 2023Wubben TJ, Chaudhury S, Watch BT, Stuckey JA, Weh E, Fernando R, Goswami M, Pawar M, Rech JC, Besirli CG.a buffer of 50 mM Tris-HCl (pH 7.4), 100 mM KCl, and 5 mM MgCl2 as previously described [8,9,17]. A SPECTROstar Omega plate reader (BMG LABTECH Inc., Cary, NC, USA) monitored absorbance at 340 nm. The MARS software suite

(18) Highly complete long-read genomes reveal pangenomic variation underlying yeast phenotypic diversityGenome ResearchMay 1, 2023Cory A. Weller, Ilya Andreev, Michael J. Chambers, Morgan Park, Joshua S. NISC Comparative Sequencing Program, Joshua S. Sadhu, Meru J. NISC Comparative Sequencing Programwith plasmids. Strain growth curves were measured in 96-well plates (Corning 3370) over 3–5 d on a SPECTROstar Omega microplate reader equipped with a plate stacker (BMG Labtech) at room temperature with 30 s of shaking at 600 RPM before

(19) Uncovering the link between the SpnIII restriction modification system and LuxS in Streptococcus pneumoniae meningitis isolatesFrontiers in Cellular and Infection MicrobiologyMay 1, 2023Hannah N. Agnew, John M. Atack, Ann R.D. Fernando, Sophie N. Waters, Mark van der Linden, Erin Smith, Andrew D. Abell, Erin B. Brazel, James C. Paton, Claudia TrappettiµM, then incubated at 37°C with 5% CO2. The OD600 was measured every 30 min for a total of 24 h in a SpectroSTAR Omega spectrophotometer (BMG Labtech). Assays were conducted in triplicate with at least two repeated independent

(20) Antioxidant, Immunostimulatory, and Anticancer Properties of Hydrolyzed Wheat Bran Mediated through Macrophages StimulationInternational journal of molecular sciencesApril 18, 2023Tomé-Sánchez I, Martínez-Villaluenga C, Martín-Diana AB, Rico D, Jiménez-Pulido I, Frias J, Dia VP.1:4:5, v:v:v) was added onto a 96-well microplate. Absorbance at 525 nm was recorded after 30 min with a Spectrostar Omega microplate reader (BMG Labtech, Ortenberg, Germany). DPPH values were calculated using a standard curve of Trolox solution

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